Hazard Control Plan Resources

Requirement for Researchers

UC San Diego researchers working with acutely toxic materials must follow the acutely toxic materials hazard control plan (HCP) in the Hazard Control Plan (HCP). Designated work area(s), appropriate administrative and engineering controls, storage practices, workspace decontamination, and PPE need to be used, as outlined by the HCP.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

An acutely toxic material is that which has been identified as causing acute health effects or long-term chronic health effects. It has the potential to cause death, disability, or immediate damage to target organs as a result of a single exposure or an exposure of short duration, even at very low concentrations. These materials are categorized based on their LC50 or LD50 values and may also be characterized as toxic gases, select agent toxins, corrosives, irritants or sensitizers.

LD50 is the median lethal dose of a chemical administered orally causing death in 50% of test animals. It should be recognized that the minimum dose causing death in some proportion of the test population will be much lower, with significant illness or harm short of lethality probably occurring at even lower doses.

LC50 is the median lethal concentration of a chemical in air administered by continuous inhalation causing death to 50% of test animals.

The following table denotes the OSHA-defined toxicity designations, for various routes of exposure. Find the LD50 on a Safety Data Sheet (SDS) or in the Registry of Toxic Effects of Chemical Substances (RTECS). Compare the LD50/LC50 to the table to determine if it is acutely toxic.

On GHS-compliant Safety Data Sheets (SDSs), acutely toxic materials can be identified via the following hazard statements:

• H300: Fatal if swallowed
• H301: Toxic if swallowed
• H310: Fatal in contact with skin
• H311: Toxic in contact with skin
• H330: Fatal if inhaled
• H331: Toxic if inhaled

Acutely Toxic Material Listings

Use the listings below to find out if your chemical inventory includes acutely toxic materials:

• List of Acutely Toxic Chemicals in Settlement Agreement (PDF)
• ATSDR Toxic Substances Portal 
• OSHA Occupational Chemical Database

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• Title 8, California Code of Regulations, Section 5191  – Occupational Exposure to Hazardous Chemicals in Laboratories (Laboratory Standard)

Requirement for Researchers

UC San Diego researchers working with an autoclave must complete an autoclave hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

An autoclave is a device that uses water, pressure and heat to create superheated steam to kill infectious agents and denature proteins. They are also used to decontaminate certain biological waste and sterilize media, instruments and labware. They use saturated steam under pressure of approximately 15 pounds per square inch to achieve a chamber temperature of at least 250° Fahrenheit (121° Celsius) for a prescribed time – usually 30-60 minutes. When used properly, an autoclave is safe and highly effective. 

Autoclave Hazards

Autoclaves use high-pressure and high-temperature steam for sterilization; despite built-in safeguards, autoclaves present potential safety risks for operators that include:

• Heat burns from hot materials and autoclave chamber walls and door.
• Steam burns from residual steam coming out from autoclave and materials on completion of cycle.
• Hot fluid scalds from boiling liquids and spillage in autoclave.
• Hand and arm injuries when closing the door.
• Cuts or lacerations from handling broken glassware.
• Body injury if there is an explosion.
• Infections from failed decontamination.
• Slips, trips and falls from wet floors.

Special Requirements

• All autoclave users must receive formal instruction on proper and safe use of the autoclaves. Contact your Safety Division Office for the Division of Biological Sciences, or a Research Assistance Program Contact in EH&S for training.
• You must fill out the autoclave user log; Facilities Maintenance relies on user logs for autoclave maintenance and repairs.
• Never set the autoclave temperature above 250° Fahrenheit (121° Celsius).
• Never autoclave biological waste unless the autoclave is specifically designated as such and part of your lab’s Biohazard Use Authorization (BUA).
• Report autoclave problems/malfunctions to Facilities Maintenance right away: 534-2930.
  • Post a note on the broken autoclave to inform other users that a service call has been placed.
  • Always remove your items promptly after your cycle is finished.
  • If something breaks or spills, it is your responsibility to clean it up.
  • When in doubt, please ask. Better to ask than to injure yourself or damage the equipment.
    • Contact Safety Division Office for the Division of Biological Sciences
    • Contact a Research Assistance Program Specialist in EH&S, or
    • Contact Facilities Maintenance 534-2930.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• UC San Diego Autoclave Overview
• Division of Biological Sciences Autoclave Information

Requirement for Researchers

UC San Diego researchers working with arsine must complete an Arsine hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Note: The Arsine HCP must be used in conjunction with the Pyrophorics HCP.

Definition

Arsine is a colorless, flammable, pyrophoric, and highly toxic gas. It has a garlic-like or fishy odor that can be detected at concentrations of 0.5ppm and above. Because arsine is nonirritating and produces no immediate symptoms, persons exposed to hazardous levels may be unaware of its presence. Exposure frequently occurs when arsine gas is generated while metals or crude ores containing arsenic impurities are treated with acid.

Special Requirements

• Consult with the Chemical Hygiene Officer before ordering arsine to ensure that you have met the requirements of the Compressed Gas Policy. There are significant fire code restrictions on the quantities of pyrophoric and highly toxic gases allowed in use or storage within research buildings. Failure to do this may lead to premature disposal of the material or costly building modifications.
• Must be SCBA trained to change out gas cylinders and the Chemical Hygiene Officer must be notified prior to cylinder change-out.

Lessons Learned

• Four Employees Injured When Exposed to Arsine Gas
• Lab Safety Gas Cylinders Incidents: Arsine Gas Release
• Tulsa Arsine Leak Incident

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• 29 CFR 1910.1200 Hazard Communication, Appendix A – Health Hazard Criteria
• CDC Occupational Health Guidelines for Chemical Hazards
• Compressed Gas: Safety Resources for Individual Gases
• Compressed Gas Policy
• Pyrophoric Materials HCP Resource drawer
• Prudent Practices in the Laboratory: Handling and Disposal of Chemicals – Laboratory Chemical Safety Summary: Arsine
• The Agency for Toxic Substances and Disease Registry’s Medical Management Guidelines for Arsine

Requirement for Researchers

UC San Diego researchers working with bromine must complete a bromine hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

Elemental bromine, a non-combustible, dense, mobile, slightly transparent reddish-brown liquid, evaporates easily at a standard temperature and pressure — which gives off an orange vapor (its color resembles nitrogen dioxide) and a strongly disagreeable smell (similar to chlorine). Odor can be detected at concentrations as low as 0.05 ppm. It is 1 of only 2 elements on the periodic table that are known to be liquids at room temperature (mercury is the other). Being less reactive than chlorine but more reactive than iodine, bromine reacts vigorously with metals, especially in the presence of water, to give bromide salts. It is also reactive towards most organic compounds, especially upon illumination conditions that favor the disassociation of the diatomic molecule into bromine radicals. It bonds easily with many elements and has a strong bleaching action.

Bromine is highly corrosive to skin and eyes, causing irritation and destruction with blister formation. If it is not removed from the skin immediately, deep-seated ulcers develop, which heal slowly. Severely painful and destructive eye burns may result from contact with either liquid or concentrated vapors of bromine. It is toxic via inhalation, causing lacrimation at concentrations below 1 ppm. Higher concentrations cause irritation and damage to the respiratory tract, and eventually pulmonary edema and/or death. Symptoms include coughing, tightness of chest, nosebleed, headache and dizziness, follow after some hours by abdominal pain, diarrhea and a measles-like rash on the trunk and extremities.

Compatibility Notes

• Bromine reacts violently with easily oxidized substances, including many organic compounds and a number of metals. Fires and explosions have been reported to occur, for example, on addition of bromine to methanol, acetaldehyde, dimethylformamide, hydrogen, ammonia, aluminum, mercury, sodium, potassium and phosphorous.

Lessons Learned

• Exposure to Bromine During a Laboratory Refrigerator Clean-Up

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• CDC NIOSH Pocket Guide to Chemical Hazards: Bromine
• The Agency for Toxic Substances and Disease Registry’s Medical Management Guidelines for Hydrogen Fluoride (HF)
• Prudent Practices in the Laboratory: Handling and Disposal of Chemicals – Laboratory Chemical Safety Summary: Bromine
• Bromine: Periodic Table Videos

Requirement for Researchers

UC San Diego researchers working with carcinogens must follow the carcinogens hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Use carcinogen stickers to label storage and work areas where known carcinogens are used. Request these stickers from RAP at ehsrap@ucsd.edu.

Definition

A select carcinogen is defined in the Laboratory Standard as a substance that meets any of the following criteria:

• It is regulated by California OSHA as a carcinogen; or
• It is listed under the category, “known to be carcinogens,” in the Annual Report on Carcinogens published by the National Toxicology Program (NTP)(U.S. DHHS, 1991); or
• It is listed under Group 1 (“carcinogenic to humans”) by the International Agency for Research on Cancer Monographs (IARC) (Volumes 1-48 and Supplements 1-8);
• It is listed in either Group 2A or 2B by IARC or under the category, “reasonably anticipated to be carcinogens” by NTP, and causes statistically significant tumor incidence in experimental animals in accordance with any of the following criteria:
  • (a) after inhalation exposure of 6 to 7 h per day, 5 days per week, for a significant portion of a lifetime to dosages of less than 10 mg/m3;
  • (b) after repeated skin application of less than 300 mg/kg of body weight per week; or
  • (c) after oral dosages of less than 50 mg/kg of body weight per day.

Carcinogen Listings

Use the listings below to find out if your chemical inventory includes known or suspected carcinogens.

• Carcinogens Regulated by Cal/OSHA (Excel) (PDF)
• National Toxicology Program's Report on Carcinogens
• International Agency for Research on Cancer Monographs (IARC)
• OSHA based Select Carcinogens list

NOTE: If any work involves any of the listed carcinogenic materials in Title 8 of the California Code of Regulations, Section 5209, you must consult with the Chemical Hygiene Officer prior to purchase. These materials have stringent requirements which need careful evaluation before acquiring. If these regulatory requirements cannot be achieved then this material cannot be purchased.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• Title 8, California Code of Regulations, Section 5191  – Occupational Exposure to Hazardous Chemicals in Laboratories (Laboratory Standard) 
• California Code of Regulations, Title 8, Section 5209 – Carcinogens
• PPM 516.5.1 Chemical Safety – Carcinogens

Requirement for Researchers

UC San Diego researchers working with a centrifuge must follow the centrifuge hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

A centrifuge is an important tool in a research lab, but can also be a dangerous instrument if not used or maintained properly. It is usually driven by an electric motor that puts an object in rotation around a fixed axis applying a potentially strong outward force. Low speed centrifuges that do not exceed 5,000 rpm are commonly found on bench tops. High speed centrifuges that do not exceed 25,000 rpm are generally floor models. Ultracentrifuges operate in excess of 100, 000 rpm.

Centrifuge Use Hazards

Serious hazards associated with centrifuging include mechanical failure and the creation of aerosols:

• Mechanical failure (e.g., rotor failure, tube, or bucket failure) can cause serious damage to equipment, laboratory space, and worker injury.
• High-speed rotor heads are prone to metal fatigue. Failure to discard rotors after a predetermined amount of use can result in dangerous and expensive rotor disintegration. Avoiding rotor failure is critically important!
• Aerosols may be created when filling centrifuge tubes, removing plugs or caps from tubes after centrifugation, removing supernatant, resuspending sedimented pellets, and by the very process of centrifugation. The greatest hazard is created if a tube breaks during centrifugation.

Lessons Learned

• Laboratory centrifuge explosion incident
• Centrifuge Explosion Damages Cornell
• Centrifugation Hazards Safety Video

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• Working With Laboratory Equipment, 6.C.6.2 Centrifuges, "Prudent Practices in the Laboratory: Handling and Disposal of Chemicals" (1995), The National Academies Press.

Requirement for Researchers

UC San Diego researchers working with flame sterilization must follow the flame sterilization hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

Flame sterilization is done to inoculating loops and straight-wires in order to kill, deactivate, or eliminate all forms of life and other biological agents.

Precautions for Safe Handling

• Clear area surrounding Bunsen burner of any combustible or flammable materials/chemicals before lighting the burner, including any bench liners or paper pads.
  • If your laboratory performs flame sterilization frequently, it is strongly recommended that you define a dedicated work space for these activities.
  • Always know where the safety equipment is (fire extinguisher, fire blanket, telephone, etc).
• Use the minimum amount of solvent needed for the sterilization.
  • The volume may not exceed 100 ml to qualify for the Chemical Safety and Surveillance Committee exemption to the LHAT PPE requirements.
• Position flammable chemicals required for the procedure away from the burner.
• Gently start the gas flow and light the burner.
• Do not wave utensil being sterilized in the air when using a flammable solvent (e.g. ethanol)
• Never leave a flame unattended.
• Ensure gas is shut off when finished with task.

Note: The use of plumbed natural gas and open flame burners within a biosafety cabinet (BSC) are strictly forbidden. See Biological Safety Cabinets: Open Flames and Flammable Gas Policy.

Alternatives

• Consider alternative flame-less methods of sterilization:
  • Glass Bead Sterilizer
  • Infrared Micro-Sterilizer
  • Electric Bunsen Burner
  • Reusable polypropylene spreaders
  • Sterile disposable loops

Learn more about alternatives to continuous flame Bunsen burners.

Lessons Learned

A recent lab fire due to flame sterilization use occurred when a researcher accidentally dropped a flamed coverslip dipped in ethanol onto a benchtop. The coverslip was then knocked into an adjacent trashcan which resulted in a fire. Quick thinking and knowing where the lab’s fire extinguisher prevented the incident from escalating into something bigger!

It is prudent to set aside an area specific for flame sterilization that is clear from immediate and neighboring combustible materials. Always ensure that the solvent container is secure and that a fire extinguisher is nearby.

Additional flame sterilization incidents: 

• Flame Sterilization Fire Incident

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.
• UCLA Employee Accident OSHA Report

Requirement for Researchers

UC San Diego researchers working with flammable liquids must complete a Flammable Liquids hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Definition

Flammable liquids have a flash point below 100° Fahrenheit (38° Celsius) regardless of the boiling point. This HCP also applies to liquids that are combustible or noncombustible (flash point at or above 100° F). Flammable solids are covered by the Pyrophorics HCP.

1. Flammable and combustible liquids ignite easily and burn with extreme rapidity.
2. Flammability is determined by the flash point of a material.
3. Flash point is the minimum temperature at which a liquid forms a vapor above its surface in sufficient concentration that it can be ignited.
4. Flammable liquids have a flash point less than 100° F. Liquids with lower flash points ignite easier.
5. The vapor burns, not the liquid itself. The rate at which a liquid produces flammable vapors depends upon its vapor pressure.
6. The vaporization rate increases as the temperature increases; therefore, flammable and combustible liquids are more hazardous at elevated temperatures than at room temperature.
7. Consider substituting less flammable solvents where possible. For example, consider replacing pentane by heptane, consider finding an alternative to carbon disulfide if at possible.

Special Requirements

• Flammable and combustible liquids present a danger of personal injury and property damage, so strict storage requirements are both essential and required by the law.
  • See Flammable and Combustible Liquids Overview for more details.
  • Always wear a flammable-resistant (FR) lab coat when handling quantities in excess of 1L.
  • Know where your lab’s fire extinguisher is located and how to use it.
    • See Fire Extinguisher Training for more information.
    • Keep only small quantities of flammable materials available for immediate use.
    • Flammable liquid containers, both dispensing and receiving vessel, must be bonded and grounded when transferring liquids.
      • See Bonding and Grounding for requirements and examples.
      • Transfer flammable and combustible liquids within a chemical fume hood when possible, or in a proper dispensing location such as a high hazard room (also called a flammable room).
      • Always store flammable liquids in a flammable cabinet when not in use.
        • Use only flammable rated refrigerators (spark-proof) if flammable liquids must be chilled or frozen.
        • Never use environment rooms (also called cold/warm rooms) to store flammable materials. Environmental rooms have many ignition sources and

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

Requirement for Researchers

UC San Diego researchers working with flash chromatography must follow the Flash Chromatography hazard control plan (HCP) in the Hazard Control Plan (HCP).

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Note: Depending on the chemicals used in flash chromatography this HCP must be cross-referenced with other applicable HCPs. Examples: Flammable Liquids HCP (methanol) and Carcinogens (methylene chloride).

Definition

Flash chromatography is a rapid form of preparative column chromatography for the separation or purification of compounds.

Flash Chromatography Hazards

• Explosions
• Flammable liquids
• Pressurized vessels
• Carcinogens
• Toxics

Lab personnel must review the UC San Diego video "Flash Chromatography 101" prior to performing your first run: 

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.
• Cole-Parmer: Chromatography Products
• Grainger: Pneumatic Regulators 
• Wikipedia: Chromatography
• Wikipedia: Silica gel

Requirement for Researchers

UC San Diego researchers working with carcinogens must follow the Gel Electrophoresis hazard control planin the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Note that if the following chemicals are used in the gel electrophoresis process they require that the Acutely Toxic Materials HCP be used in conjunction with the Gel Electrophoresis HCP:

• Ethidium Bromide
• Acrylamide

Definition

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. There are differing types of electrophoresis units but horizontal electrophoresis is the most commonly used unit for separating DNA molecules on agarose gels, which is a non-toxic polysaccharide derivative of agar. Vertical electrophoresis is used with a polyacrylamide gel medium, which is created from acrylamide and bis-acrylamide powder.

Evaluate the Hazards

• Chemical and reproductive hazards:
  • Acrylamide (suspected carcinogen, reproductive toxin and neurotoxin)
  • Ethidium bromide (possible mutagen and requires appropriate disposal)
  • Molecule staining agents (methylene blue, silver stain, Coomassie Brilliant Blue)
  • Flammable solvents
  • Thermal hazards:
    • Liquefying gels can produce superheated liquids
• Radiological hazards:
  • UV light boxes
• Electrical shock:
  • Potential hazardous current of up to 2000 volts and 80 milliamps
  • Handling conductive liquids around electricity

Injury Prevention

• Avoid handling chemical powders; purchase premixed solutions or pre-made gels instead of making them.
• Use less hazardous and environmentally friendly alternatives to ethidium bromide such as GelRed, GelGreen (Biotium),SYBR Green and EZ Vision DNA Dye.
• Never mix ethidium bromide into the buffer solution prior to heating it in the microwave.
• Always let superheated liquids cool before handling and use insulated gloves.
• Always inspect the electrophoresis unit and power supply for damage, missing parts, exposed connectors, leaks and liquid spills prior to use.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• CDC Occupational Health Guidelines for Chemical Hazards
• Electrophoresis Safety
• Prudent Practices in the Laboratory (PDF)

Requirement for Researchers

UC San Diego researchers working with a glove box or anaerobic chamber must follow the glove box hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with the equipment. Information on this page is supplementary and is not intended to replace the approved HCP.

Note: Depending on the chemicals used in a glove box or anaerobic chamber this HCP must be cross referenced with other applicable HCPs. Examples: compressed gases and pyrophorics.

Definition

Glove boxes, glove bags, and anaerobic chambers are equipment used for applications involving hazardous materials and/or manipulating substances that require a controlled atmosphere or vacuum. Some materials or processes may be sensitive to ambient environments and need to be worked with under isolated conditions. Examples of such materials include water reactive and pyrophoric chemicals, radioactive isotopes, nanomaterials and biological materials (e.g., cell cultures, viruses or DNA).

Glove Box Hazards

• Over and under pressurization of glove box
• Valve failure
• Ejection of materials when used in conjunction with mechanical, pneumatic or vacuum systems
• Simultaneous operation by two or more laboratory personnel
• High voltage
• Use of incompatible chemicals and/or gases
• Inappropriate ventilation or exhaustion of vapors
• Glove breaches and failures

 Anaerobic Chamber Hazards

• Hydrogen gas use:
  • It is colorless, tasteless, odorless and highly flammable in concentrations between 4%-75% by volume.
  • It may spontaneously ignite in the air and burn with an almost invisible flame.
  • High levels of hydrogen gas or incorrect mixtures of hydrogen gas introduced during purge cycles may cause explosions.
  • Even at low concentrations of hydrogen gas, the use of heated catalysts, incubators, fans and stirrers inside the chamber can serve as ignition sources.
• Glove breaches and failures

Lessons Learned

• Hydrogen Explosion in University Biochemistry Laboratory
• Hydrogen Explosion in Microbiological Chamber
• Incorrect Flammable Gas Cylinder Attached to Anaerobic Chamber
• Mixed Gas Explosion at University of Hawaii
• Worker Contaminated While Working in a Glove Box

Resources

• Engineering controls to help manage your materials.
• Personal protective equipment (PPE) to help protect you while you work.

References

• CDC Occupational Health Guidelines for Chemical Hazards
• Prudent Practices in the Laboratory (PDF)

Requirement for Researchers

UC San Diego researchers working with any chemical that is considered a health hazard must complete a health hazard hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material.

Definition

Health hazard chemicals are those that are classified as causing toxicity: acute health effects, chronic health effects, irritancy or sensitivity. In some cases these materials may also have carcinogenic properties. OSHA defines a health hazard as a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established significant principles that acute or chronic health effects may occur in exposed employees. The broad definition OSHA uses to define hazardous chemicals includes not only generic chemicals but also paints, cleaning compounds, inks, dyes and other common substances. This is why materials such as borax, Bromophenol blue and cholesterol require a health hazard HCP.

Health Effect Definition

Toxic: A chemical that causes adverse health effects in humans or animals upon exposure. This definition excludes highly toxic materials that are captured in the separate acutely toxic materials HCP.

Acute health effects: Immediate effects caused to humans or animals by the direct, short-term, exposure of a chemical.

Chronic health effects: Long-term effects cause to humans or animals by exposure of a chemical over time; typically weeks, months or years after the exposure occurred.

Irritant: A chemical that causes irritation, either reversible or irreversible, to skin or living tissue at the site of contact; resulting in redness, swelling, itching, or a rash at the site of contact.

Sensitizer: A chemical that upon repeated exposure causes a severe adverse reaction. Allergens are also included in this grouping.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• 29 CFR 1910.1200 Hazard Communication, Appendix A – Health Hazard Criteria
• CDC Occupational Health Guidelines for Chemical Hazards
• Prudent Practices in the Laboratory (PDF)

Requirement for Researchers

UC San Diego researchers working with hydrofluoric acid must complete a hydrofluoric acid Hazard Control Plan (HCP) in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Note: If work involving hydrogen fluoride gas or flourine gas is conducted then the compressed gas HCP must be used in conjunction with the Hydrofluoric Acid HCP.

Definition

Hydrogen fluoride (HF) is a colorless, fuming liquid or gas with a strong, irritating odor. It is usually shipped in steel cylinders as a compressed gas. Hydrogen fluoride readily dissolves in water to form colorless hydrofluoric acid solutions; dilute solutions are visibly indistinguishable from water. It fumes at concentrations greater than 48%. Although it is non-flammable, its corrosive action on metals can result in the formation of hydrogen in containers and piping, which creates a fire and explosion hazard.

Liquid hydrofluoric acid is one of the most corrosive acids and can produce serious health effects by any route of exposure. Concentrated HF solutions can cause severe, deep, and disfiguring burns that are extremely painful and slow to heal. These effects are due to the fluoride ion’s aggressive, destructive penetration of tissues, which also binds with calcium in the body. This results in tissue destruction, decalcification of bone, cardiac arrhythmia, liver and kidney damage. Exposure to dilute solutions (concentrations less than 20%) may cause few or no symptoms at first, but may cause severe pain later if not quickly washed off. Calcium gluconate gel will bind to the fluoride ions and prevent further tissue destruction, but it must be applied quickly (even if burns have not been felt) to be effective. Burns may not be painful or visible for several hours and even moderate exposure to concentrated HF can result in fatality.

Breathing HF gas or the vapor from hydrofluoric acid can cause rapid death from throat swelling or from chemical burns to the lungs. Acute symptoms of HF inhalation include coughing, choking, chest tightness, chills, fever and cyanosis. Anyone suspecting of having inhaled HF should seek immediate medical attention and observation of pulmonary effects. 

Special Requirements

• Labs having hydrofluoric acid in their inventories MUST have calcium gluconate gel within the expiration date accessible in their lab’s first-aid kit. Contact an EH&S Research Assistance Program specialist for supplies.
  • Laboratory personnel should be trained in first-aid procedures for HF exposure before beginning any HF related work.
• Consult the Chemical Hygiene Safety Officer if work involves large quantities or heating.
• Hydrofluoric acid attacks glass, concrete, and some metals (especially cast iron and alloys containing silica), organic materials such as leather, natural rubber, wood and human tissue. Never store HF is a glass container.

Lessons Learned

• Fatality Due to Acute Fluoride Poisoning Following Dermal Contact with Hydrofluoric Acid in Palynology Laboratory
• Hydrofluoric Acid: What You Need to Know
• First Aid For a Unique Acid, HF: A Sequel

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

Requirement for Researchers

UC San Diego researchers working with microtomes must follow an approved hazard control plan in the Hazard Control Plan (HCP) application. This HCP must be preapproved by the principal investigator prior to beginning any work with this equipment. Adhering to the HCP can significantly reduce the risk of possible injury.

Definition

A microtome, also referred to as a histome or cryostat, is a device that cuts extremely thin sections of tissue for microscopic study. They present a significant risk during operation or cleaning due to very sharp blades.

Evaluate the Hazards

• Cuts and body injuries can occur when:
  • Placing or removing blocks from the block holder: the blade is in the blade holder and the wheel lock is NOT on; when the block holder sweeps down towards the blade and fingers are in the way.
  • Changing blades.
  • Cleaning the microtome while the blade is in place.
  • Grabbing a ribbon from the blade with fingers.
  • Wiping the face of the block in the block holder while blade is in place.

Injury Prevention

• Avoid using excessive force with blades.
• Utilize the blade guard.
• Always lock the wheel when exchanging blocks.
• Make it your practice to use handles with blades.
• Slowly and carefully change blades:
  • Always remove blade before cleaning microtome; do NOT leave the blade in a microtome when not in use.
  • Use appropriate tools (such as forceps) when retrieving materials near a blade so hands remain clear;
• Use a clamping tool to remove and install blades.
• Always discard blades in a sharps container immediately.
• Maintain a minimum 1" (or 2.54 centimeters) clearance between your hands and the blade.

Required Mentorship for All Users

• Important: Follow a mentor strategy before beginning work until proficiency using a microtome has been demonstrated.
  • The first time observe.
  • The second time work cooperatively.
  • The third time conduct the microtome work in the presence of a mentor.
  • The fourth time become a mentor — provide feedback to your principal investigator regarding the HCP.

Lessons Learned

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.
• Microtome Safety Precautions and Training Document (Word) (PDF)
• Cryostat Safety Precautions and Training Document (Word) (PDF)
• Safety Checklist for Operating a Cryostat or Microtome (PDF) (Small printable PDF version)

References

• Title 8, Standard 3558 – Microtomes (Manual, Semiautomatic and Automatic)

Requirement for Researchers

UC San Diego researchers working with opioids/opiates must complete an Opioids and Opiates Hazard Control Plan (HCP) in the Hazard Control Plan (HCP) application.

This HCP must be by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Never work alone when working with hazardous chemicals.

Definition

Opioids are a class of drugs with analgesic (pain-relieving) properties and have both legal (clinical, research) and illegal (drug abuse) uses. Some opioids, called Opiates, are natural compounds derived from the opium poppy plant and include Heroin, Morphine, and Codeine. Other types of opioids are synthetic and are manufactured to mimic the effects of opiates using a similar chemical structure, including Fentanyl and its analogs.

Overdose/exposure to opioids can have serious side effects and be life threatening. Signs of overdose/exposure include stupor, changes in pupil size, clammy skin, cyanosis, coma, and respiratory failure leading to death. The presence of a triad of symptoms such as coma, pinpoint pupils, and respiratory depression strongly suggests opioid intoxication. If signs of an overdose/exposure are present, Naloxone is an FDA-approved medicine that can be administered to reverse the effects of opioids in the body. Depending on the strength of the opioid and severity of the overdose/expose, multiple doses of Naloxone may be required to counteract the effect of the overdose. Naloxone is intended for an immediate emergency treatment but does not replace professional emergency care. Having Naloxone in the laboratory, and laboratory staff trained to administer the drug, can provide timely assistance to an individual affected by an opioid exposure. 

Special Requirements

• Work with materials regulated as controlled substances requires labs to have an approved Controlled Substance Use Authorization (CSUA) with individual personnel approvals through the UC San Diego Controlled Substances Program (CSP) before work can begin.
  • See Determine if a Chemical is a Controlled Substance or contact the Controlled Substances Program for more information.
• Labs working with potent opioids MUST have Naloxone within the expiration date accessible in their lab’s first-aid kit. Contact the Controlled Substances Program for supplies.
  • Potent opioids include powdered or liquid Fentanyl and its analogs, such as Alfentanil, Remifentanil, and Sufentanil. Patch formulations of opioids present a reduced risk of serious exposure and do not require labs to have Naloxone or complete the corresponding training video.
  • Laboratory personnel should be familiar with the location of the lab’s first aid kit and where Naloxone is stored. Additionally, laboratory personnel who conduct research with potent opioids should complete the How to Administer Naloxone training in UC Learning Center before beginning any potent opioid related work.
  • These requirements are in place to reduce the risk of opioid exposure to researchers and to provide additional safety measures in the event of exposure. Exposure to opioids can have serious side effects and be life threatening.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.
• Learn more about the Controlled Substances Program.
• Health Promotion Services - Naloxone Distribution Project for information and training resources.

References

• Centers for Disease Control and Prevention - Opioid Basics
• Centers for Disease Control and Prevention - Fentanyl and Work
• National Institute on Drug Abuse - Fentanyl DrugFacts
• National Institute on Drug Abuse - Naloxone DrugFacts
• Centers for Disease Control and Prevention - Fentanyl: Incapacitating Agent

Requirement for Researchers

UC San Diego researchers working with organic peroxides must follow the organic peroxides hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Never work alone when working with hazardous chemicals.

Requirement for Researchers

UC San Diego researchers working with piranha solution must follow an approved hazard control planin the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material.

Never work alone when working with hazardous chemicals.

Definition

Piranha solution is a mixture of sulfuric acid and hydrogen peroxide used to remove organic residues from substrates. Traditional piranha solution is a 3:1 mixture of sulfuric acid and 30% hydrogen peroxide. Some protocols call for as much as 7:1 mixtures.

Piranha solution (also known as piranha etch) is extremely energetic, highly corrosive, and potentially explosive. Handle with extreme caution.

Special Precautions

• Caution: Mixing hot piranha with organic compounds may cause an explosion. This includes acetone, photoresist, isopropyl alcohol and nylon.
• See Berkeley lesson learned with Piranha Solution.

Required Containers, Caps, and Labels

• Important: UC San Diego requires researchers to use the specific containers, vented caps, and labels listed below for piranha solution. Other pressure generating waste streams also require vented waste containers (examples: base piranha [ammonium hydroxide and hydrogen peroxide], aqua regia, organic solvents containing residual concentrated nitric acid, etc.).

Get Free Required Containers, Vented Caps, and Labels from EH&S:

• Clear poly-coated acid bottle (see image in ‘Equipment Cited in Hazard Control Plan’ below).
  • Free from EH&S: Contact the EH&S Environmental Management Facility.
  • Or order from vendor: Order Sigma Aldrich, product #Z568686, case of 12.
  • Vented cap
    • Free from EH&S: Contact the EH&S Environmental Management Facility.
    • Or order from vendor: Performance Systematix Inc., product #38439C4/RC.
  • Specific labels (see image at right) to be affixed to all piranha solution processing containers
    • Free from EH&S: Contact the EH&S Environmental Management Facility.
    • See Laboratory Safety Labels for a piranha solution label you can print out yourself.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

Requirements for Researchers

UC San Diego researchers working with potentially explosive materials must follow an approved hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Never work alone when working with hazardous chemicals.

Definition

Potentially explosive materials (PEMs) are particularly dangerous because they may explode if subjected to heat, light, friction or mechanical shock. Shock-sensitive chemicals also become increasingly sensitive with age. These materials all require careful management due to their capability of posing as a serious threat to health and safety.

PEMs include peroxidizable organic chemicals.

Storage and Inventory Management

• Label potentially explosive materials clearly and promptly upon receipt or synthesis (including refillable dispensing containers).
  • Date received (ex: 1/1/2010 RCVD, initials)
  • Date opened (ex: 1/1/2010 OPND, initials)
• Review your inventory frequently to prevent PEMs from becoming unsafe.
• Use a first-in, first-out inventory — A system using the oldest chemicals first should be implemented to avoid accumulation and degradation of older chemicals and their containers.
• Unless the manufacturer added an inhibitor, closed containers of shock sensitive materials should be discarded after 1 year and open containers discarded within 6 months of opening. Review the label and SDS to determine if the chemical is shock sensitive.
• Keep explosive chemicals away from all ignition sources such as open flames, hot surfaces, spark sources, and direct sunlight.

If You Discover Expired or Old PEMs

• DO NOT HANDLE ANY PEM forming material that appears suspicious (crystal formation, discoloration or visible layering)
• Inform your principal investigator immediately.
• Inform colleagues in the work area about the hazard.
• Contact the Chemical Hygiene Officer and your Departmental Safety Officer as soon as possible.
• Label and isolate the cabinet or area: “Do Not Handle — Potentially Explosive Material”.
• Contact EH&S Environmental Management Facility, (858) 534-2753, promptly for proper disposal.
  • See How to Request a Hazardous Waste Collection.

Commonly used chemicals that can become an explosion hazard under certain conditions:

• Hydrated picric acid that becomes dry or becomes contaminated with metals which form metal picrate salts.
• Sodium amide that reacts with air or moisture to form superoxides, as evidenced by yellow or brown discoloration.
• Certain alkyl nitrates (e.g., butyl nitrate or propyl nitrate) that become contaminated with nitrogen oxides.
• Certain normally stable perchlorates (e.g., pyridium perchlorate or tetraethylammonium perchlorate) that becomes unstable at elevated temperatures.
• Organic chemicals that form peroxides through exposure to air or light: Common Laboratory Chemicals That Form Organic Peroxides.

NOTE: Most explosions occur while purifying or distilling mixtures. Therefore, use extreme caution before concentrating or purifying any mixture that may contain an explosive chemical.

• Acetone + chloroform in the presence of a base
• Acetylene + copper, silver, mercury, or their salts
• Ammonia (including aqueous solutions) Cl_2, Br₂, or I₂
• Carbon disulfide + sodium azide
• Chlorine + an alcohol
• Chloroform or carbon tetrachloride + powdered Al or Mg
• Decolorization carbon + an oxidizing agent
• Diethyl ether + chlorine (including a chlorine atmosphere)
• Dimethyl sulfoxide + an acyl halide, SoCl₂, or POCL₃
• Dimethyl sulfoxide + CrO₃
• Ethanol + calcium hypochlorite
• Ethanol + silver nitrate
• Nitric acid + acetic anhydride or acetic acid
• Picric acid + a heavy metal, such as Pb, Hg, or Ag
• Silver oxide + ammonia + ethanol
• Sodium + a chlorinated hydrocarbon
• Sodium hypochlorite + an amine

Requirements for Researchers

UC San Diego researchers working with pyrophoric materials must follow an approved hazard control plan in the Hazard Control Plan (HCP) application. This HCP must be preapproved by the principal investigator prior to beginning any work with this material. 

Never work alone when working with hazardous chemicals. 

Researchers who work with pyrophoric materials outside of an inert atmosphere glove box are required to wear basic lab attire and the following:

• Protective eyewear
• Flame Resistant Lab Coats
• Fire Resistant Glove Liners coupled with chemical-resistant outer gloves
• FR Glove Liners (Ansell 70-200 Kevlar Liner gloves) are provided by EH&S.  FR Glove Liner Training is mandatory and available through UC Learning Center.
• Neoprene chemical-resistant outer gloves preferred (Ansell 25-201 NeoTouch® neoprene gloves).

Requirement for Researchers

UC San Diego researchers working with any chemical that is considered a reproductive hazard must complete a reproductive hazard hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material.

Definition

A reproductive hazard is a substance that has an adverse effect on the reproductive capabilities including chromosomal damage, effects on fetuses, male and female fertility and the development of offspring. These hazards may cause problems such as infertility, miscarriage and birth defects.

Use the listings below to find out if your chemical inventory includes reproductive hazards:

• Reproductive Hazards List Compiled by UC San Diego (PDF)

• Chemicals Known to the State to Cause Cancer or Reproductive Toxicity (PDF), California Environmental Protection Agency
  
  • The lists above serve as guides and are not all inclusive. Review the Safety Data Sheet for other materials not listed.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.
• Preventing Reproductive Hazards in the Workplace 
• The Effects of Workplace Hazards on Female Reproductive Health
• The Effects of Workplace Hazards on Male Reproductive Health

References

• Title 8, California Code of Regulations, Section 5191  – Occupational Exposure to Hazardous Chemicals in Laboratories (Laboratory Standard link)

Requirement for Researchers

UC San Diego researchers working with sodium azide must complete a sodium azide hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this page is supplementary and is not intended to replace the approved HCP.

Definition

Sodium azide is an inorganic, rapidly acting, highly toxic, odorless white solid chemical. It is also very reactive, decomposing explosively upon shock, heating, or friction. Sodium azide is usually used in dilute solutions as a chemical preservative for samples and stock solutions in research labs and in clinical applications. In other research applications, such as synthesis, the use of pure sodium azide and solutions of 5% or greater present serious physical and health hazards; however, health hazards apply regardless of usage. It is acutely toxic by all routes of exposure and should be handled with extreme care. It may be fatal if swallowed or absorbed through the skin. Symptoms of exposure are similar to those of cyanide and even small amounts may include hypotension, hypothermia, headache, shortness of breath, faintness, cough, skin burns and blisters, and convulsions with permanent damage targeting the heart and brain.

Special Handling Requirements

• Avoid disturbing sodium azide chemical containers when not actively using and avoid exposing concentrated sodium azide to friction, heat or shock.
• Never store sodium azide in metal containers or on metal shelves. The chemical will react to form explosive metal azides.
• Do not use metal items, such as metal spatulas, to manipulate. Use Teflon spatulas instead.
• Do not use a HEPA vacuum for cleaning up sodium azide — the chemical could react explosively with the metal inside the vacuum.
• Do not pour sodium azide solutions down the drain, even at low concentrations — the chemical could react explosively with the metal inside the drain piping.
  • Hematology equipment that uses sodium azide and generates liquid waste must not be piped to the drain. The buildup of sodium azide salts in the piping system have led to serious explosions in the plumbing infrastructure. For proper and safe disposal of hemostatis system equipment waste, please contact the EH&S Chemical Hygiene Officer.
• Sodium azide should not be allowed to come into contact with the following, as sensitive and/or unstable compounds can be formed: 
  • Heavy metals or their salts (including barium, lead, and copper)
  • Nitric acid and other acids (acids can also form the highly toxic and volatile hydrazoic acid).
  • Chlorinated solvents such as dichloromethane and chloroform, bromine, and dimethyl sulfate.

Lessons Learned

• USC Sodium Azide Explosion 

Resources

• Engineering controls to help manage your materials.
• Personal protective equipment (PPE) to help protect you while you work.
• CDC: Facts About Sodium Azide
• ATF’s Federal Register List of Explosive Materials (PDF)

Definition

Corrosive chemicals are substances that cause the destruction of living tissue at the site of contact, or are highly corrosive to steel. Corrosive chemicals can be solid, liquids, or gases and can affect the eyes, skin and respiratory tract. These materials are probably the most common toxic substances encountered in the laboratory and can be especially dangerous because their effects on tissue generally takes place very rapidly.

Strong corrosives have a pH of 2 or lower or a pH of 12.5 or higher. Most acids are liquids and most bases are solids. Acids react with many metals and can be in themselves, strong oxidizing agents. They also have the ability to produce fires or explosions by combination with combustible materials. It is imperative to follow all strong corrosive incompatible and reactivity segregation guidelines.

Special Handling

• Always be vigilant about wearing laboratory PPE; corrosive chemicals pose serious hazards to dermal and eye exposures.
• Never store corrosive materials above eye level.
• Always add acids and bases to water; NEVER water to acids and bases.
• When mixing corrosive solids with water, always add slowly the corrosive solid to the water stirring continuously. Cooling may also be necessary.
• If the potential exists for explosion or a high-thermal reaction, additional shielding must be utilized. This may include working in a fume hood or a glove box, using a portable shield and donning a face shield.
• If there is a possibility of corrosive chemical dust generation or fumes/vapors, conduct the work in a fume hood. Always ensure that there is adequate ventilation.
• Always store corrosive materials away from heat, flames, oxidizers and water sources.
• Always segregate acids and bases in storage.
• Keep chemical containers closed and store in an acid-resistant secondary container.
• If you need to transport corrosive chemicals utilize a bottle jacket; Marketplace offers 4L/1G sizes:
  • Grainer Catalog Number: 1YNJ4
  • Sigma-Aldrich Catalog Number: Z194441
  • Fisher Catalog Number: 1798579
  • VWR Catalog Number: 56610-100
    

Acid/ Base Baths

• Potential Hazards
  • Inhalation of noxious corrosive fumes.
  • Skin contact of concentrated acids/bases.
  • Uncontrolled release of corrosive chemicals down the sink drain/environment.
• Generation and disposal of an acid/ base bath must always have the approval of the PI.
  • Always wear the mandatory laboratory PPE.
  • Additional PPE may include a chemical splash apron, rubber gloves, and face shield.
• Clearly label acid/ base bath containers
  • • All containers, even those containing just water or bleach must be clearly labeled (not just the lids).
    • Include the chemical’s hazard on the container; i.e.: “10% bleach, Corrosive”.
    • Consider using different colored containers for acid and base baths.
• Lessons learned
  • Accidental mixing of bleach in acid bath.
  • The Strong Corrosives HCP is one of the most frequently used HCPs across campus; don’t be complacent about corrosive chemical safety!

Bottle jacket for transporting corrosive chemicals.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

Requirement for Researchers

UC San Diego researchers working with any chemical that is considered a strong oxidizer must complete a strong oxidizers hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Never work alone when working with hazardous chemicals.

Strong Oxidizer Listings

Strong oxidizer HCPs come in two possible categories of HCP templates: 

1) CSSC Approved also known as material specific HCPs. These templates are approved by UC San Diego's Chemical Safety & Surveillance Committee (CSSC) for work with materials identified as high-hazard chemicals, such as:

• Bromine
• Chlorine
• Fluorine
• Hydrogen peroxide
• Osmium tetroxide
• Piranha solution

2) EH&S Reviewed also known as group templates. These are standard HCP templates for work with thousands of other chemicals grouped by hazard classification:

• Strong oxidizers
• Compressed gas

Definition

Oxidizing chemicals are materials that provide oxygen to cause/contribute to the combustion of other materials at room temperature or with slight heating. Strong oxidizing agents are capable of creating fire and explosive reactions when combined with combustible, organic or easily oxidized materials. This class of chemicals includes peroxides, chlorates, perchlorates, nitrates and permanganates.

In addition to the physical hazards, the health hazards of strong oxidizers can be severe. The combustion products of oxidizer-fed fires are generally much more toxic than the combustion products of the combustible material itself in air and can cause potentially lethal chemical pulmonary edema even with brief exposures. Oxidizers are also generally corrosive; tissues such as lung, skin and eyes are at risk, which will depend on the oxidizer and its concentration. Skin exposure can result in dangerous burns, but dermatitis is more common. Eyes are much more sensitive to exposure.

Example of Strong Oxidizers

Special Handling

• Heating perchloric acid must only be done in a perchloric acid designed/designated fume hood that is marked as for use ONLY with perchloric acid and not any other materials unless approved by the Chemical Safety Officer.
• Store oxidizers away from organic, flammable, dehydrating, or reducing agents.
• Never store oxidizers in wooden cabinets or on wooden shelves.
• Provide secondary containment for strong oxidizing acids such as perchloric and chromic acid.
• Do not use corks or rubber stoppers.
• Reaction vessels containing oxidizing material should be heated in a mantle or sand bath. Oil baths should not be used.
• Do not let combustible solids such as paper towels, lab coats and clothing become contaminated with oxidizers. Should this happen, immediately soak and rinse well with water to remove oxidizer.
• Do not use potentially reactive oxidizer mixtures outside of accepted temperature ranges. The additional heat may initiate violent or even explosive reactions.
• Perchloric Acid Guidelines
• Picric Acid Guidelines

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• Title 8, California Code of Regulations, Section 5191  — Occupational Exposure to Hazardous Chemicals in Laboratories (Laboratory Standard)
• Hazards of Strong Oxidizers

Requirement for Researchers

UC San Diego researchers working with trifluoroacetic acid must complete a trifluoroacetic acid hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

Note: Trifluoroacetic Acid (TFA) in this HCP refers to Neat TFA. Any dilutions of TFA are handled using standard chemical safety practices.

Definition

Trifluoroacetic acid is a noncombustible colorless liquids with a sharp pungent odor and is highly corrosive. It is miscible in water. Contact of the liquid with the skin, eyes, and mucous membranes can cause severe burns, and ingestions can result in serious damage to the digestive tract. TFA vapor is highly irritating of the eyes and respiratory tract, and inhalation of high concentrations can lead to severe destruction of the upper respiratory tract and may be fatal as a result of pulmonary edema. Symptoms of overexposure to TFA vapor include a burning feeling, coughing, headache, nausea and vomiting.

Lessons Learned

• Hydrofluoric Acid Burn from Trifluoroacetic Acid
• OSHA: Employee Suffers Acid Burns From Trifluoroacetic Acid

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

Requirement for Researchers

UC San Diego researchers working with any chemical that is considered a water reactive hazard must complete a water reactive hazard control plan in the Hazard Control Plan (HCP) application.

This HCP must be preapproved by the principal investigator prior to beginning any work with this material. Information on this Blink page is supplementary and is not intended to replace the approved HCP.

• When using piranha solution you must follow the EH&S approved piranha solution HCP (above).
• Never work alone when working with hazardous chemicals.

Definition

Water reactive materials can react violently with water or atmospheric moisture to produce gas and heat. The most common water reactive chemicals include sodium, potassium, lithium metals and aluminum alkyls. The risks associate with a specific chemical depends on its reactivity and the nature of the gaseous product (flammable, toxic or both). The mutual production of flammable gas and heat can lead to spontaneous ignition or explosion. Typical gasses produced are: H2, CH4, H2S, NH3, PH3, HCN, HF, HCl, HF, HI, SO2, SO3. Prior to working with any water reactive chemicals you must identify which gas may be formed in case of exposure to water and learn the risks associate with this gas.

• Remember that water reactive material may present additional hazards. These chemicals may also be classified as corrosives, acutely toxic materials.

NOTE: When handling air-sensitive materials, be prepared for the unexpected. For example, at least one extra set of clean, dry syringes and needles or double-tipped needles should always be available in case the first set of equipment becomes plugged. When working with these air-sensitive reagents keep in mind that these solutions should never be allowed to come in contact with the atmosphere. The reaction rate of solid material (and therefore heat and gas generation) depends on the material’s surface area; therefore smaller particle size increases the hazards associated with these materials.

Quenching — Any unused or unwanted water reactive materials must be destroyed by following the specific destruction procedure(s) included in the Lab Specific Instructions section of the HCP. Design a quenching scheme for residual materials prior to use. Begin quenching with a low-reactivity quenching agent and slowly add more.

Safety Precautions

• Safety shielding is required anytime there is a risk of explosion, splash hazard or a highly exothermic reaction. This includes using the fume hood sash to the lowest feasible position and/or a portable shield.
• Work under an inert atmosphere (e.g., argon, nitrogen) in a glove box when possible.
• Work away from water sources or where there is a potential of a water splash.
• Keep materials in a dry place such as a desiccator, a dry box, or glove box.
• Store under an inert atmosphere when not in use.
• Store in a location separated by bases, oxidizing and other incompatible materials.
• Never return excess chemicals to the original container.
• Never use water to extinguish fires caused by water reactive materials; use a Class D extinguisher only.
• Always thoroughly inspect the area for residual reactive materials at the end of each project.

Resources

• Engineering Controls to help manage your materials.
• Personal Protective Equipment (PPE) to help protect you while you work.

References

• Title 8, California Code of Regulations, Section 5191  — Occupational Exposure to Hazardous Chemicals in Laboratories (Laboratory Standard)