National Institutes of Health (NIH) Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules (NIH Guidelines) states that with some exceptions, experimental use of Escherichia coli (E. coli) K-12 strain and its derivatives are exempt from the requirements of the NIH Guidelines. This exempt status allows for faster review and approval of the Biohazard Use Authorization.
Below is a list of some commonly used strains of E. coli, and the NIH Guidelines category applicable to those strains.
The following E. coli laboratory strains are K12 or derived from K-12 and therefore most research utilizing these strains is exempt from the NIH Guidelines. (RG1, Exempt, NIH Guideline III-F, F-8, Appendix C-II)
Ancestral E. coli K-12
Strain Designation |
Origin or Collection |
58 |
Stanford Strain |
679 |
Stanford strain |
WG1 |
Wisconsin strain |
E. coli K-12 Derivatives
Strain Designation |
Origin or Collection |
Strain Designation |
Origin or Collection |
5K |
Lab strain |
JC9387 |
Lab strain |
58 |
Lab strain |
JM83 |
Lab strain |
58-161 |
Lab strain |
JM101 |
Lab strain |
AB284 |
Lab strain |
KP7600 |
Lab strain |
AB311 |
Lab strain |
LE392 |
Lab strain |
AG1 |
Lab strain |
M15 |
Lab strain |
C600 |
Lab strain |
MB408 |
Lab strain |
Cavalli Hfr |
Lab strain |
MG1655 |
Lab strain |
DH1 |
Lab strain |
Novablue |
Novagen |
Dh5-alpha |
Lab strain |
P678 |
Lab strain |
DP50 |
Lab strain |
PA 309 |
Lab strain |
EMG2 |
Lab strain |
REG-12 |
Lab strain |
EPI100-T1R |
Lab strain |
S17-1 |
Lab strain |
H1443 |
Lab strain |
SCS-110 |
Stratagene |
HB101 |
Lab strain |
SM10 |
Lab strain |
Hfr3000 |
Lab strain |
STBL2 |
Invitrogen |
Hfr 3000 X74 |
Lab strain |
STBL3 |
Invitrogen |
HMS 174 |
Novegen |
SURE |
Lab strain |
JM109 |
Lab strain |
TB1 |
NEB |
TG1 |
Lab strain |
WA704 |
Lab strain |
TOP10 |
Invitrogen |
W1485 |
Lab strain |
W1485 |
Lab strain |
W3110 |
Lab strain |
W208 |
Lab strain |
XL1-Blue |
Stratagene |
W3110 |
Lab strain |
XL10-Gold |
Stratagene |
W945 |
Lab strain |
XLOLR |
Stratagene |
WA704 |
Lab strain |
Y10 |
Lab Strain |
WG1 |
Lab strain |
YN2980 |
Lab Strain |
E. coli NOT Derived from K-12
The following E. coli laboratory strains are not derived from K-12 and therefore any research utilizing these strains is not exempt from the NIH Guidelines. Non-K12 E. coli strains (RG1, not exempt, NIH Guideline III-E)
Strain Designation |
Origin or Collection |
Strain Designation |
Origin or Collection |
B |
Laboratory strain |
K5808 |
Laboratory strain |
B-3 |
Laboratory strain |
Mach1 |
Invitrogen |
B/R |
Laboratory strain |
Nissile 1917 |
Laboratory strain |
BL21 |
Novagen |
Rosetta |
Novagen |
BL23 |
NEB |
REG-811 |
Laboratory strain |
C |
Laboratory strain |
TOPP |
Stratagene |
C41 |
Sigma Aldrich |
W |
Laboratory strain |
C43 |
Sigma Aldrich |
25922 |
Laboratory strain |
FDA strain Seattle 1946 |
Laboratory strain |
|
Pathogenic E. coli strains (not exempt, III-D NIH Guidelines)
Strain Designation |
E. coli, all strains bearing K1 antigen |
E. coli enteroaggregative strain (EAEC) |
E. coli enterohaemorrhagic strain (EHEC) |
E. coli enteroinvasive strain (EIEC) |
E. coli enteropathogenic strain (EPEC) |
E. coli enterotoxigenic strain (ETEC) |
E. coli Shiga toxin-producing E. coli (O157:H7) |
References
- EcoliWiki
- NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules (NIH Guidelines)
- National Center for Biotechnology Information (NCBI) Taxonomy Browser: E.coli genome
- NCBI Rapid and accurate identification of Escherichia coli K-12 strains
- The University of Iowa: E. coli Derivatives
- Virginia Polytechnic Institute and State University: E. coli Strain Information
- Yale: The Coli Genetic Stock Center, E. coli Genetic Resources