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Escherichia coli Strains and NIH Guidelines

Learn which K-12 strains of E. coli are exempt from NIH Guidelines.

National Institutes of Health (NIH) Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules (NIH Guidelines) states that with some exceptions, experimental use of Escherichia coli (E. coli) K-12 strain and its derivatives are exempt from the requirements of the NIH Guidelines.  This exempt status allows for faster review and approval of the Biohazard Use Authorization.

Below is a list of some commonly used strains of E. coli, and the NIH Guidelines category applicable to those strains.

The following E. coli laboratory strains are K12 or derived from K-12 and therefore most research utilizing these strains is exempt from the NIH Guidelines. (RG1, Exempt, NIH Guideline III-F, F-8, Appendix C-II)

Ancestral E. coli K-12 

Strain Designation

Origin or Collection

58

Stanford Strain

679

Stanford strain

WG1

Wisconsin strain

E. coli K-12 Derivatives

Strain Designation

Origin or Collection

Strain Designation

Origin or Collection

5K

Lab strain

JC9387

Lab strain

58

Lab strain

JM83

Lab strain

58-161

Lab strain

JM101

Lab strain

AB284

Lab strain

KP7600

Lab strain

AB311

Lab strain

LE392

Lab strain

AG1

Lab strain

M15

Lab strain

C600

Lab strain

MB408

Lab strain

Cavalli Hfr

Lab strain

MG1655

Lab strain

DH1

Lab strain

Novablue

Novagen

Dh5-alpha

Lab strain

P678

Lab strain

DP50

Lab strain

PA 309

Lab strain

EMG2

Lab strain

REG-12

Lab strain

EPI100-T1R

Lab strain

S17-1

Lab strain

H1443

Lab strain

SCS-110

Stratagene

HB101

Lab strain

SM10

Lab strain

Hfr3000

Lab strain

STBL2

Invitrogen

Hfr 3000 X74

Lab strain

STBL3

Invitrogen

HMS 174

Novegen

SURE

Lab strain

JM109

Lab strain

TB1

NEB

TG1

Lab strain

WA704

Lab strain

TOP10

Invitrogen

W1485

Lab strain

W1485

Lab strain

W3110

Lab strain

W208

Lab strain

XL1-Blue

Stratagene

W3110

Lab strain

XL10-Gold

Stratagene

W945

Lab strain

XLOLR

Stratagene

WA704

Lab strain

Y10

Lab Strain

WG1

Lab strain

YN2980

Lab Strain

E. coli NOT Derived from K-12

The following E. coli laboratory strains are not derived from K-12 and therefore any research utilizing these strains is not exempt from the NIH Guidelines. Non-K12 E. coli strains (RG1, not exempt, NIH Guideline III-E)

Strain Designation

Origin or Collection

Strain Designation

Origin or Collection

B

Laboratory strain

K5808

Laboratory strain

B-3

Laboratory strain

Mach1

Invitrogen

B/R 

Laboratory strain

Nissile 1917

Laboratory strain

BL21

Novagen

Rosetta

Novagen

BL23

NEB

REG-811

Laboratory strain

C

Laboratory strain

TOPP

Stratagene

C41

Sigma Aldrich

W

Laboratory strain

C43

Sigma Aldrich

25922

Laboratory strain

FDA strain Seattle 1946

Laboratory strain

 

Pathogenic E. coli strains (not exempt, III-D NIH Guidelines)

Strain Designation

E. coli, all strains bearing K1 antigen

E. coli enteroaggregative strain (EAEC)

E. coli enterohaemorrhagic strain (EHEC)

E. coli enteroinvasive strain (EIEC)

E. coli enteropathogenic strain (EPEC)

E. coli enterotoxigenic strain (ETEC)

E. coli Shiga toxin-producing E. coli (O157:H7)

References