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Read about different autoclave cycles and access correct procedures for safe and effective autoclaving.
Autoclaving, sometimes called steam sterilization, is the use of pressurized steam to killinfectious agents and denature proteins. This kind of "wet heat" is considered the most dependablemethod of sterilizing laboratory equipment and decontaminating biohazardous waste.
Autoclaves do not remove chemical contamination.
When used properly, autoclaves are safe and highly effective. For operating procedures, read:
There are 2 basic autoclave cycles:
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| Gravity or "fast exhaust" | Dry goods, glassware, etc. | This cycle charges the chamber with steam and holds it at a set pressure and temperature for a set period of time. At the end of the cycle, a valve opens and the chamber rapidly returns to atmospheric pressure. Drying time may also be added to the cycle. |
| Liquid or "slow exhaust" | Liquids | This cycle prevents sterilized liquids from boiling. Steam is exhausted slowly at the end of the cycle, allowing the liquids (which will be super-heated) to cool. |
Other decontamination methods — including dry heat, ultraviolet or ionizing radiation, and liquid, gas, or vapor disinfection — are not a proper substitute for autoclaving or incineration before disposing of biohazardous material.
Notice: Disposal of hazardous waste using sinks, intentional evaporation, or as regular trash is against the law. Campus laboratories must abide by strict state and federal waste disposal requirements. You may be held liable for violations of applicable laws.