| What to do |
How to do it |
| 1 |
Prepare the primary vacuum flask with bleach. |
- Add bleach to the primary vacuum flask to equal 10% of the maximum collection volume. Maximum collection volume should be no more than two-thirds full.
|
| 2 |
Label the flask. |
- Label the flask as follows: "Tissue culture media disinfected with bleach 9:1"
|
| 3 |
Protect the vacuum system with a filter. |
- Insert an in-line hydrophobic HEPA filter before the vacuum outlet.
In-line HEPA filters
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| 4 |
Add the tissue culture media to be disinfected. |
- Aspirate tissue culture media into bleach in the flask to make a final concentration of 10% bleach solution (9 parts tissue culture media:1 part straight bleach).
- After reaching maximum volume, allow a minimum 30-minute contact time.
- Empty the flask into the laboratory sink.
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